Effects of an Enterococcus faecium‐based probiotic on growth performance and health of Pirarucu,Arapaima gigas     

Natalino da Costa Sousa  Mrcia Valria Silva do Couto  Higo Andrade Abe  Peterson Emmanuel Guimares Paixo  Carlos Alberto Martins Cordeiro  Emilly Monteiro Lopes  Jonathan Stuart Ready  Gabriel Fernandes Alves Jesus  Mauricio Latera Martins  Jos Luiz Pereira Mourio  Paulo Csar Falanghe Carneiro  Alexandre Nizio Maria  Rodrigo Yudi Fujimoto 《Aquaculture Research》2019,50(12):3720-3728

Intensive fish farming has resulted in an increased concern for disease outbreaks. Probiotic use is one of the strategies being developed to improve fish health and productivity. Measures of probiotic colonization, growth performance, haematological characteristics and parasite load were used to evaluate the effect of diets supplemented with Enterococcus faecium on growth and health of Arapaima gigas juveniles. A completely randomized design with four treatments (diet with E. faecium at 1 × 10⁶ CFU/g and 1 × 10⁸ CFU/g, control diet and diet with the culture medium MRS) and three replicates was used. Ninety‐six Arapaima juveniles were distributed in 12 cages fed with the specified diet for 68 days. Colonization of the intestinal tract by lactic acid bacteria reduced the total number of heterotrophic bacteria in fish fed with probiotics compared to controls. Fish fed a supplemented diet containing 1 × 10⁸ CFU/g presented higher values of weight gain, survival and fish growth uniformity, and lower values of feed conversion ratio. The prevalence of Trichodina sp. could have affected the survival of fish in the control group. Reduction in parasite load and an increase in haematocrit, the number of erythrocytes, thrombocytes, neutrophils and monocytes were also observed in fish fed the diet containing 1 × 10⁸ CFU/g. Enterococcus faecium presented a probiotic effect in A. gigas juveniles and can be recommended for use at a concentration of 1 × 10⁸ CFU/g to modify the gut microbiota, improve growth performance and haematology and reduce parasitic load.  相似文献   

Artificially induced tetraploid masu salmon have the ability to form primordial germ cells     

Suzu Sakao  Takafumi Fujimoto  Terumasa Kobayashi  Goro Yoshizaki  Etsuro Yamaha  Katsutoshi Arai 《Fisheries Science》2009,75(4):993-1000

Diploid gametes generated with tetraploid animals are a stepping stone to improving chromosome manipulation techniques. However, artificially induced tetraploid individuals generally die soon after hatching. Diploid gametes could be induced by in vivo cultures of tetraploid primordial germ cells (PGCs) through germ-line chimera. In the present study, characteristics of PGCs were studied in inviable tetraploid masu salmon, Oncorhynchus masou. Histological observation of tetraploid embryos revealed that the same or smaller numbers of PGCs were observed and they migrate into the genital ridges as did diploid PGCs during gonadogenesis. By whole-mount in situ hybridization using vasa messenger RNA (mRNA), 4–35 vasa-positive signals were detected in a pair of genital ridges of tetraploids. By cytological observation of genital ridge cell suspensions, several large round cells were observed, some of which extended pseudopodia. They also contained large nuclei and round granules in their cytoplasm, characteristics of PGCs. As the results suggest that inviable artificial tetraploids have PGCs, we expect to achieve diploid gamete production through surrogate propagation and tetraploid fish production.  相似文献   

Plasma estrone, estradiol-17 beta, and progesterone levels during late pregnancy and parturition in dairy cattle     

T Sawada  E Kimura  Y Fujimoto  H Matsunaga  J Mori 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(3):654-658

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Effects of feeding condensed barley distillers soluble on growth rate,ruminal fermentation,plasma metabolites,and myofiber properties of the longissimus thoracis muscle in Japanese Black calves     

Katsuhiko Tsuruoka  Takami Kurahara  Hidenobu Kanamaru  Hideyuki Takahashi  Takafumi Gotoh 《Animal Science Journal》2019,90(5):637-648

This study evaluated the effects of condensed barley distillers soluble (CBDS) on growth rate, rumen fermentation, plasma metabolite, and myofiber properties, and gene expression related to metabolism in the skeletal muscles of Japanese Black calves, compared with soybean meal and corn. Twenty‐four calves were divided into four groups: fed 5% CBDS based on the hay dry matter weight (low CBDS) and fed soybean meal and corn at the same nutrition level (control); and fed 15% CBDS based on the hay dry matter weight (high CBDS) and fed soybean meal and corn at the same nutrition level (high soy). The daily gain was larger in the low (p = 0.08) and high (p < 0.05) CBDS groups compared with the control group. In the CBDS‐fed groups, plasma β‐hydroxybutyric acid concentrations were significantly higher at 6 months of age (p < 0.05), the percentage of type I myofibers was significantly lower and their diameters were significantly larger at 9 months of age (p < 0.05), and carnitine palmitoyltransferase 1b mRNA expression was significantly lower (p < 0.05) and citrate synthase mRNA expression tended to be lower (low; p = 0.06, high; p = 0.05) compared with control group. Thus, feeding CBDS promotes growth and leads to animals with more glycolytic and less oxidative muscle metabolism.  相似文献   

Effects of a rice diet and phytase addition on growth performance,tissue weights,phosphorus and nitrogen retention,and on liver threonine dehydrogenase,malic enzyme and fatty acid synthase activities in broiler chicks          下载免费PDF全文

Hiroya Fujimoto  Nozomi Fujita  Ryozo Takada 《Animal Science Journal》2018,89(5):770-776

This experiment was conducted to clarify the nutritional functions of rice and phytase addition for broiler chicks. Thirty‐six 7‐day‐old male chicks (ROSS 308 strain) were assigned to one of the four treatment groups: corn‐ or rice‐based diet groups and each diet with added phytase (2000 phytase units/kg diet) groups (corn + P or rice + P groups). The non‐phytate phosphorus (npP) content in the diets with added phytase was approximately half of the requirement. Body weight gain and feed intake in the rice group was significantly higher than those in the corn group. Breast and thigh muscle weights and nitrogen retention in the rice group were significantly higher than that in the corn group. Although the efficiency of phosphorus retention (%) in the corn + P group was significantly higher than that in the corn group, no significant difference was observed between the rice and rice + P groups. Liver threonine dehydrogenase activity in the corn group was significantly higher than in the other three groups. These results indicate that rice is superior to corn as a starter diet in broiler chicks, and that phytase action in the rice‐based diet was less than that in the corn‐based diet.  相似文献   

Agent-based modeling of complex social–ecological feedback loops to assess multi-dimensional trade-offs in dryland ecosystem services     

Takafumi Miyasaka  Quang Bao Le  Toshiya Okuro  Xueyong Zhao  Kazuhiko Takeuchi 《Landscape Ecology》2017,32(4):707-727

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Growth of large and small forms of pond smelt Hypomesus nipponensis in Lake Kasumigaura, Japan     

Takafumi Kudo  Kenya Mizuguchi 《Fisheries Science》2000,66(3):432-441

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Perception and valuation of Paddy Field Dam functions by rural communities: a CVM approach     

Oishi  Takafumi  Nakano  Ryota  Matsuno  Yutaka 《Paddy and Water Environment》2019,17(3):383-390

Paddy and Water Environment - In recent years, Paddy Field Dams have received recognition as a measure to alleviate flooding due to torrential rains. Paddy Field Dams have been in practice in...  相似文献   

Regulation of programmed death ligand 1 expression by interferon-γ and tumour necrosis factor-α in canine tumour cell lines     

Ryo Owaki  Tatsuya Deguchi  Satoru Konnai  Naoya Maekawa  Tomohiro Okagawa  Kenji Hosoya  Sangho Kim  Takafumi Sunaga  Masahiro Okumura 《Veterinary and comparative oncology》2023,21(2):279-290

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Cucurbita-5,23-diene-3beta,25-diol from Sicana odorifera     

Nakano S  Fujimoto Y  Takaishi Y  Osorio C  Duque C 《Fitoterapia》2004,75(6):609-611

The spectral data of a new triterpene, cucurbita-5,23-diene-3beta,25-diol, isolated from the seeds of Sicana odorifera, are reported.  相似文献